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Detecting Base-Pair Mismatches and Modifications in Polynucleotides through Base Flipping
Invention SummaryThis technology proposes a novel way of detecting localized conformational mutations in DNA, also known as single nucleotide polymorphisms (SNPs), through utilizing a protein nanopore alpha hemolysin. Using this technology, base pair mismatches can be identified by measuring local conformational changes in the DNA molecule, from which...
Published: 3/19/2019
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Inventor(s):
Henry White
,
Cynthia Burrows
,
Robert Johnson
,
Aaron Fleming
Keywords(s):
Category(s):
Research Tools & Reagents
Nickel-Based Reagents for Detecting DNA and DNA-Protein Contacts **Value Express**
Patent Abstract The invention relates to a compound comprising a labeled nickel complex (FIG. 1) that is used to detect and purify nucleic acid sequences by forming an adduct between a specific nucleic acid base and the labeled nickel complex. The adduct can also be purified through a separation process, such as affinity chromatography Intellectual...
Published: 3/22/2019
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Inventor(s):
Steven Rokita
,
Cynthia Burrows
Keywords(s):
Category(s):
Research Tools & Reagents
,
Reagents
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