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Novel Primer Design for Quantitative Allele-Specific PCR

Invention SummaryThis invention provides a novel primer design, incorporating site-specific mismatches and locked nucleic acids, for real-time quantitative allele-specific PCR (AS-PCR), resulting in improved sensitivity, specificity and reproducibility of detection. This method provides greatly improved quantitation of low-level polymorphisms and alleles,...

Published: 1/29/2020 | Inventor(s): Josef Prchal, Roberto Nussenzweig, Sabina Swierczek

Keywords(s): Diagnostics, Molecular, Nucleic Acid Diagnostics

Category(s): Diagnostics, Molecular