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Novel Primer Design for Quantitative Allele-Specific PCR
Invention SummaryThis invention provides a novel primer design, incorporating site-specific mismatches and locked nucleic acids, for real-time quantitative allele-specific PCR (AS-PCR), resulting in improved sensitivity, specificity and reproducibility of detection. This method provides greatly improved quantitation of low-level polymorphisms and alleles,...
Published: 1/29/2020
|
Inventor(s):
Josef Prchal
,
Roberto Nussenzweig
,
Sabina Swierczek
Keywords(s):
Diagnostics
,
Molecular
,
Nucleic Acid Diagnostics
Category(s):
Diagnostics
,
Molecular
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